Preliminary evaluation of urine EIA for detection of antibodies to HIV - 1 in urine specimens of Thai HIV infected patients
By: S Kohreanudom1, S Dettrairat2, K Faithong2
1Bureau of AIDS, TB and STI, Nonthaburi, Thailand; 2Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai , Thailand
Background: Testing of antibodies to HIV - 1 with blood specimens is the gold standard method for identifying HIV infected persons. Urine specimens may be alternative to blood specimens and facilitate surveillance of HIV infection.
Methods: Urine specimens were randomly collected from 100 Thai HIV-1 infected patients being treated with ARV drugs and 59 normal healthy persons. Specimens were aliquot and kept at 4-10 °C until testing. All urine specimens were tested for antibodies to HIV-1 by Calypte HIV-1 urine EIA as the test instruction. Moreover, all urine specimens were also tested for antibodies to HIV-1 with simple particle agglutination test for blood specimens with slightly modified procedure. Specimens giving discordance results by 2 methods were repeatedly tested with the same specimens. Sensitivity and specificity were calculated using the final test results compared to HIV status of the subjects.
Results: Urine EIA gave sensitivity of 98% (98/100) with urine specimens collected from HIV infected patients. By testing with urine specimens collected from normal healthy persons, the specificity was 100 % (59/59). Unsurprisingly, particle agglutination test for blood specimens, showed lower in both sensitivity (86%,86/100) and specificity (98%, 58/59) than urine EIA.
Conclusion: By using specimens from Thai HIV-infected patients, urine EIA has high sensitivity and specificity. Testing urine specimens with urine EIA may be benefit for surveillance of HIV infection in Thailand.
Calypte ist bei, jetzt fehlt noch der offizielle Ritterschlag, und dann
kanns los gehen !!!
Gruß werweiß
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euch gefragt warum dieser kursverlauf.ich meine die amis haben doch sicher infos von der konferenz.vielleicht ist calypte noch nicht erwähnt worden und deshalb der abverkauf?die auf einen kracher dort gehofft haben und den kurs gepusht haben, gehen jetzt wieder raus?