Schon heute am ersten Tag der Aids2004 referieren wichtige Thai-Offizielle (siehe unten) über Calyptes Urin-Tests ! Beachtet die "Conclusions" (Schlußfolgerungen) !
Topic: Preliminary evaluation of urine EIA for detection of antibodies to HIV - 1 in urine specimens of Thai HIV infected patients
By: S Kohreanudom1, S Dettrairat2, K Faithong2 1Bureau of AIDS, TB and STI, Nonthaburi, Thailand; 2Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai , Thailand
Background: Testing of antibodies to HIV - 1 with blood specimens is the gold standard method for identifying HIV infected persons. Urine specimens may be alternative to blood specimens and facilitate surveillance of HIV infection. Methods: Urine specimens were randomly collected from 100 Thai HIV-1 infected patients being treated with ARV drugs and 59 normal healthy persons. Specimens were aliquot and kept at 4-10 °C until testing. All urine specimens were tested for antibodies to HIV-1 by Calypte HIV-1 urine EIA as the test instruction. Moreover, all urine specimens were also tested for antibodies to HIV-1 with simple particle agglutination test for blood specimens with slightly modified procedure. Specimens giving discordance results by 2 methods were repeatedly tested with the same specimens. Sensitivity and specificity were calculated using the final test results compared to HIV status of the subjects.
Results: Urine EIA gave sensitivity of 98% (98/100) with urine specimens collected from HIV infected patients. By testing with urine specimens collected from normal healthy persons, the specificity was 100 % (59/59). Unsurprisingly, particle agglutination test for blood specimens, showed lower in both sensitivity (86%,86/100) and specificity (98%, 58/59) than urine EIA.
Conclusion: By using specimens from Thai HIV-infected patients, urine EIA has high sensitivity and specificity. Testing urine specimens with urine EIA may be benefit for surveillance of HIV infection in Thailand.
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